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do you know the five major requirements for the design of a P3 clean laboratory?

Mar 09, 2019

The construction of the laboratory generally has to consider many factors. In particular, the design requirements of the P3 clean laboratory are very strict, and only high standards can produce high quality engineering. So let's take a look at the main five major requirements for the design of the P3 clean laboratory!

lab furniture-07

Site selection problem

The tertiary biosafety laboratory can be housed in a building with other uses, but must be self-contained. The area is separated from the public corridor or public part by an isolation gate.

2. Plane layout problem

a) The core area of the tertiary biosafety laboratory includes the experimental room and the buffer room connected to it.

b) The buffer room forms a passage into the experimental room. Two interlocking doors must be provided. When one of the doors is opened, the other door is automatically closed. If an electric interlock is used, both doors must be open when the power is off. 2. A second change can be made in the buffer room.

c) When the laboratory ventilation system does not have an automatic control device, the buffer room area should not be too large, and should not exceed one-eighth of the experimental room area.

d) The installation position of the Class II or Class III biosafety cabinet should be away from the entrance of the laboratory, avoiding the area where the staff frequently moves, and is conducive to the formation of the airflow pattern from the “clean” area to the “contaminated” area.

3. Enclosure structure problems

a) The inner surface of the enclosure (including the buffer room) must be smooth, corrosion-resistant, waterproof, easy to disinfect and clean. All gaps must be reliably sealed.

b) All doors in the laboratory can be automatically closed.

c) No window shall be provided except the observation window. The observation window must be a sealed structure, and the glass used is not broken glass.

d) The ground should be free of leakage, smooth but not slippery. Do not use grounded tiles such as floor tiles and terrazzo.

e) The angle between the ceiling, the floor and the wall is round and reliable, and insects and rats should be prevented from drilling into the wall during construction.

Ventilation and air conditioning problem

a) A separate ventilation and air conditioning system must be installed to control the direction of the laboratory airflow and pressure gradient. The system must ensure that the indoor air is not filtered from other parts of the laboratory or the gap, except that it is efficiently filtered through the exhaust duct. Discharge to the outside; at the same time ensure that the airflow in the laboratory flows from the “clean” area to the “contaminated” area. The layout of the air inlet and exhaust vent should reduce the dead space in the experimental area to a small extent.

b) Ventilation and air conditioning system is a straight exhaust system, and part of the return air system shall not be used.

c) Environmental parameters: Relative to the outside of the laboratory, the internal pressure of the laboratory is maintained. The relative pressure between the experiments is preferably -30Pa ~ -40Pa, and the relative pressure between the buffers is -15Pa ~ -20Pa. Temperature and humidity should be controlled in the comfort range of the human body, or according to the process requirements. The air cleanliness in the laboratory is suitable for the seven to eight grades defined in GB 50073-2001 "Code for Design of Clean Plants". The artificial lighting of the room should be uniform, not dazzling, and the illumination should be no less than 500lx.

d) In order to ensure that the airflow in the laboratory flows from the “clean” area to the “contaminated” area, the layout of the exhaust vents on both sides should not be used in the laboratory. The ventilation design of the upper row should not be used. Biosafety The internally efficiently filtered air discharged from the cabinet can be directly discharged to the atmosphere through the exhaust duct of the system, or can be sent to the exhaust system of the building. The pressure balance between the biosafety cabinet and the exhaust system should be ensured.

e) The inlet air of the laboratory should be filtered by primary, middle and high efficiency.

f) The exhaust of the laboratory must be directly filtered into the air at a speed of not less than 12m/s after high efficiency filtration or other methods. The exhaust vent should be away from the air inlet of the system. The exhaust after treatment can also be used. Discharge into the exhaust duct of the building, but must not be returned to any part of the building.

g) Inlet and exhaust air Efficient filters must be installed in the tuyere of the laboratory on the enclosure to avoid contamination of the duct.

h) In the ventilation system of the laboratory, a gas-tight regulator valve shall be installed at the inlet and exhaust manifolds, and if necessary, it may be completely closed for indoor chemical fumigation.

i) All components used in the laboratory ventilation system must be airtight. The high efficiency filter used must not be a wood frame.

j) The fan should be installed to start the automatic interlock device to ensure that the fan is opened after the fan is started. When the fan is turned off, the fan is turned off after the fan is turned off.

k) Do not install split air conditioners in the laboratory.

5. Safety devices and special equipment problems

a) A Class II or Class III biosafety cabinet must be installed in the main laboratory. The installation location should meet the requirements in d).

b) Continuous flow centrifuges or other equipment that may generate aerosols should be placed in physical containment equipment that is capable of filtering the aerosols that may be produced by the high efficiency filter. It must be set up in the laboratory. Exhaust air from all other exhaust devices (fume hoods, exhaust hoods, etc.) must be filtered through a high-efficiency filter before discharge. The indoor layout should facilitate the formation of airflow from the “clean” zone to the “contaminated” zone. .

c) An autoclave or other disinfection device that does not produce steam must be installed in the laboratory.

d) The transfer window should be set between the experimental room and the outside. The double doors of the transfer window should not be opened at the same time. The physical disinfection device should be set in the transfer window. The infectious material must be placed in a closed container before being passed through the transfer window.

e) The pressure display alarm device must be set at a prominent position at the entrance of the laboratory to display the negative pressure condition between the experimental room and the buffer room. When the negative pressure indication deviates from the preset interval, it must be able to pass the sound and light to the personnel inside and outside the laboratory. An alarm is issued. The display of the airflow resistance of the air supply and exhaust high efficiency filter can be added to the device.

f) The power can not be cut off during the start-up work of the laboratory. Dual power supply should be used. If it is difficult to achieve, install backup power or uninterruptible power supply that can be automatically switched during power failure, for key equipment (biological safety cabinet, fume hood, exhaust) Cover and lighting, etc.) power supply.

g) There can be a sink in the buffer room: the water supply door of the sink must be pedal, elbow or automatic switch. If the sink is located in the main laboratory, the sewer must be separated from the building's sewer line and have obvious signs. The sewage must be disinfected. The sink should only be used for hand washing. Do not pour any infectious materials inward. The water supply pipe must be installed with anti-reflux devices. Do not install floor drains in the laboratory.

After a small series of explanations, do you understand a lot? Then there is a need to build a P3 clean laboratory partners can consult us at any time, we look forward to your visit.