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How is the secondary biological laboratory safe?

Oct 17, 2018

Depending on the degree of hazard of the biological factors being manipulated and the protective measures taken, the relevant protective barriers must meet the requirements of the corresponding biosafety level.

Warning sign:

1. The laboratory entrance, laboratory entrance, laboratory operation room, equipment and other equipment are attached with corresponding warning signs to list various potential hazards in the laboratory.

2. Biohazard identification:

The background color of the logo is yellow, the text is black, and the biological danger is secondary. Non-workers are prohibited from entering.

3. Chemical hazard identification:

Based on the hazard characteristics and status of existing hazardous chemicals in the laboratory, the following markings are made:

Explosives, flammable gases, flammable liquids, flammable solids, drugs, corrosives, carcinogenic teratogenic products.

Physical protection barrier:

Laboratory biosafety must be equipped with a primary physical barrier that includes: biosafety equipment at all levels and personal protective equipment (primary protective barrier). The facility's facility structure and ventilation design form a secondary physical barrier (secondary barrier). Operations that can cause spills, spills, and aerosols, including: centrifugation, grinding, agitation, strong shock mixing, ultrasonic disruption, opening of containers containing infectious materials, nasal injections of animals, collection of infected animals, and tissues that hatch eggs. Class II biosafety cabinets and physical protection equipment are used.

Facilities and equipment

1. Laboratory facilities (secondary protective barriers) are in the building and the laboratory is isolated from the general area.

(1) Building structure and layout

The building has eight seismic resistance, anti-rat, insect-proof and anti-theft.

The laboratory in the building is equipped with an access control system that prohibits entry by unrelated personnel.

A cabinet is placed at the entrance of the laboratory to separate the personal clothing from the experimental clothes.

The laboratory has safe passages and emergency exits and is clearly marked.

(2) airtightness and inner surface

The walls, ceilings and floors of the laboratory are smooth, easy to clean, free from dust, water, non-condensable vapors, chemical and disinfectant corrosion. The surface is flat and non-slip. The work surface is easy to clean, waterproof, heat resistant, organic solvents, acids, alkalis and common disinfectants damage and corrosion. All doors in the laboratory can be closed; the laboratory outlet has signs that are clearly identifiable in the dark.

(3) Air conditioning

Each laboratory is equipped with air conditioning.

(4) Lighting is suitable for all activities in the room.

(5) Reliable power supply.

The refrigerator is equipped with a backup power supply.

2. Safety equipment (primary protective barrier)

(1) Protective clothing

(2) Protective gloves

(3) Biological safety cabinet

The handling of infectious materials, such as bacterial isolation, tissue culture, chicken embryo inoculation, and collection of animal body fluids, should be performed in a Class II biosafety cabinet. The biosafety cabinet is inspected once every 12 months by the manufacturer. Each time the biosafety cabinet is used, it should be observed whether it is operating normally and fill in the usage record.

(4) Autoclave

Autoclaving is a common physical means of killing microorganisms. The cultures and wastes used are sterilized by a high pressure steam sterilizer before treatment.

(5) First aid kit

The laboratory must be equipped with a first aid kit. The first aid kit should include at least: disinfectant, disinfectant cotton, disposable rubber gloves, sterile wound dressings/gauze, tape, bandages, tweezers, scissors, etc.

(6) Fire equipment

The laboratory shall be equipped with applicable fire-fighting equipment such as fire extinguishers.

(7) Eyewash device

The eyewash device is configured in the laboratory work area.

General requirements for personal protective equipment

Any personal protective equipment used in the laboratory shall comply with the requirements of relevant national standards. On the basis of biohazard assessment, select appropriate personal protective equipment according to different levels of protection requirements, and develop procedures to control the selection, use and maintenance of personal protective equipment. . .

1. Laboratory protective clothing:

The laboratory should ensure that adequate protective clothing is available. Protective clothing should be disinfected and cleaned in time. When potentially dangerous materials are highly likely to spill on workers, plastic aprons or liquid-proof long suits, if necessary, should also be worn with other personal protective equipment such as gloves, goggles, masks, head and face shields. Wait. When wearing a one-piece protective suit, be careful to wear pants first and then wear tops. After the experiment, first take off the shirt, then take off the pants, grab the top of the coat and pull it up when undressing, and drill out from the head. Contaminated protective clothing should be treated harmlessly.

2. Gloves:

Gloves should be available for use in the laboratory to prevent biohazard, chemicals, radiation contamination, cold and heat, product contamination, stab wounds, abrasions and animal bites. Gloves shall conform to the requirements of comfort, fit, flexibility, grip, abrasion, tie and tear resistance in accordance with the nature of the operation performed and shall provide adequate protection against the hazards involved. When wearing gloves, wear gloves without leakage, dry hands; replace gloves when torn, damaged or suspected of internal contamination. Disinfect the gloves with disinfectant before removing the gloves. Then, hold the palm of the other hand and pull the palm of the hand outward to make the contaminated part inside. Gloves after use should be treated harmlessly.

3. Shoes:

The experimental shoes should be comfortable and the soles should be non-slip.

4. Mask:

Choose a suitable mask and check for damage and cleanliness before wearing. When you wear it, you must pinch your nose to keep it close to your skin to prevent air leakage.

5. Hat:

A hat must be worn when handling infectious materials.

6. Glasses:

Wear protective goggles when necessary.