Microbiology laboratories generally include: preparation room, microbial culture room, instrument disinfection and cleaning room, balance room, pure water room, detection room, strain room, storage room and so on. Microbiology laboratories vary greatly in the composition and scale of laboratories, depending on the field of work (food, medicine, medical care, etc.) and nature (teaching, production, research, monitoring, etc.).
First, the layout of the microbiology laboratory
The microbiology laboratory should be set up as an independent area, separated from other laboratories, with access control at the entrance, and non-related personnel should not enter. The rooms are reasonably laid out according to the work content, which is convenient for work and does not affect each other. A centralized locker room is provided at the entrance, and a plurality of rooms (eg, a mold culture room, a bacteria culture room, a solid culture, a liquid culture, etc.) can be set in the culture room according to different culture conditions and types.
Clean Laboratory: Self-contained laboratories, arranged at the corners of the laboratory, using sealed doors to restrict people's access, placing rooms with cleanliness requirements in areas where there is little interference with the personnel, and setting up auxiliary rooms outside. Considering the microbiological test operating procedure, the detection chamber is adjacent to the scrubbing sterilization chamber and the culturing chamber to facilitate the separation of human flow and logistics. In order to control personnel's access (person flow), only one sealed door enters the main clean area of the microbiology laboratory, the operator enters the logistics corridor and then enters the preparation room, and passes through a more buffered entry area into the operating area; Air shower, buffer into local hundred level laboratory. Logistics is realized by transfer windows. The exhaust outlet is equipped with a high-efficiency filter, the air outlet is equipped with a high-efficiency filtration static pressure box, the indoor delivery exhaust air is sent to the upper and lower rows, and the indoor exhaust air is unilaterally arranged without obstructions. The excess pressure valve automatically adjusts the pressure in the room to maintain a positive pressure clean state.
Second, laboratory design and decoration
1, must provide enough space for the safe operation, cleaning and maintenance of the laboratory.
2. Laboratory walls, ceilings, and floors should be smooth, easy to clean, leak-proof, and resistant to chemicals and disinfectants. The floor should be non-slip.
3. The test bench should be waterproof and resistant to disinfectants, acids, alkalis, organic solvents and moderate heat.
4, should ensure that all activities in the laboratory lighting, to avoid unnecessary reflection and flash.
5, laboratory equipment should be sturdy and durable, between the bench, cabinets and other equipment and underneath to ensure there is enough space for cleaning.
6. There should be sufficient storage space for items to be used at any time, and another long-term storage room should be provided outside the working area of the laboratory.
7. Sufficient space and facilities should be provided for safe operation and storage of solvents, compressed gas, and liquefied gas.
8. The door of the laboratory should have a visible window and an appropriate fire rating should be achieved, preferably closed automatically.
9. The safety system should include fire protection, emergency power supply, emergency shower and eyewash facilities.
10. Reliable and adequate power supply and emergency lighting to ensure the safety of personnel leaving the laboratory.
11. The design, construction and installation of the equipment should be easy to operate, easy to maintain, clean, remove contamination and carry out quality inspections. Avoid using glass and other fragile items as much as possible.
Third, microbiology laboratory equipment
1, sterile room and clean bench: is the core part of the laboratory, mainly to provide protection for the sample to ensure the accuracy of the experimental results. The sterile room provides a relatively sterile environment for microbiological experiments through air purification and space sterilization. The main equipments in the sterile room include transmission windows, ultraviolet light, strict sterile room equipped with plenum, and clean bench: The clean bench is easy to use and is divided into horizontal flow and vertical flow according to the wind direction.
2, incubator: electric constant temperature incubator, water type constant temperature incubator, biochemical incubator, constant temperature and humidity box, anaerobic incubator, light incubator and so on.
3, drying oven: electric drying oven, vacuum drying oven.
4, sterilizer: high pressure steam sterilization pot, dry heat sterilizer.
5, balance: ± 0.1mg analytical balance, ± 10mg electronic balance.
6, the microscope: biological microscope, stereo microscope, fluorescence microscope, inverted microscope and so on.
7, spectrophotometer: visible spectrophotometer, UV spectrophotometer for quantitative analysis.
8, acidity meter: used for the solution PH value determination.
9, conductivity meter: mainly used to measure water quality.
10, refrigerators: low temperature refrigerators, ultra-low temperature refrigerators, ordinary refrigerators, vertical refrigerators and so on.
11, ice machine.
12, electric steamer, ultra-pure water machine.
13, constant temperature water bath, mixer, ultrasonic cleaning machine, electric heating plate.
14, nitrogen device.
15. Centrifuge: low-speed centrifuge, desktop high-speed centrifuge.
16, microscopic image analysis system.
17. Bacterial identification system.