First, meaning and purpose
Microbiology is the science of studying microbes and their life activities. Microorganisms have many kinds of characteristics such as various types, small volume, many types of metabolism, high metabolic intensity, fast growth and reproduction, and easy variation. It is widely distributed in air, water, soil, human body, animal and plant, and it is independent or parasitic. Most microorganisms are beneficial to humans, animals and plants, especially because they have a close relationship with human life and have a great impact on industrial and agricultural production, human living environment and health. With the new curriculum reform, the number of middle school microbes in secondary school teaching has also increased, especially the "microbial laboratory training" experiment is a subject experiment of the human science edition of the standard high school curriculum standard experimental textbook biological selection I. Microbiology experiments are not only comprehensive and operative, but also require students to have strong aseptic skills and biosafety awareness. It is of great significance to train students' theory, practical ability, ability to analyze and solve problems, and scientific thinking.
Second, the basic requirements of the microbiology laboratory A preparation room preparation room for the preparation of culture media and sample processing. Indoors are equipped with reagent cabinets, storage cabinets or materials for counters, test benches, electric stoves, refrigerators, water and sewage, power supplies, etc.
The washing chamber washing chamber is used for washing dishes and the like. Pathogenic microorganisms are sometimes present because used utensils have been contaminated with microorganisms. Therefore, the washing chamber is set up as conditions permit. Indoors should be equipped with heaters, steamers, basins and buckets for washing utensils, as well as various bottle brushes, decontamination powder, soap, washing powder, etc.
Third, the sterilization room sterilization room is mainly used for sterilization of the medium and sterilization of various instruments. The room should be equipped with high-pressure steam sterilizer, oven and other sterilization equipment and facilities.
4. Sterile room The sterile room is also called the inoculation room. It is a special laboratory for aseptic operation such as system inoculation and purification of bacteria. In the microbe, the inoculation transplantation of the strain is a major operation. The characteristic of this operation is to ensure the pure species of the strain and prevent the contamination of the bacteria. In the air of the general environment, due to the presence of many dust and bacteria, it is easy to cause pollution and interferes greatly with the inoculation work.
1. Setting up the sterile room The sterile room should be set according to the principles of economy and science. The basic requirements are as follows:
(1) The sterile room should have two inner and outer rooms, the inner room is a sterile room, and the outer part is a buffer room. The volume of the room should not be too large to facilitate air sterilization. The small inner area is 2×2.5=5m2, the outer area is 1×2=2m2, and the height is 2.5m or less. All should have ceiling.
(2) Sliding doors shall be provided in the interior to reduce the fluctuation of the air. The door shall be located at a position far from the workbench; the outer door shall also be used for sliding doors, and shall be located at a distance from the inside.
(3) A small window shall be opened on the wall or "sparing fan" between the inner and outer compartments to make the necessary passages for the inside and outside of the inoculation process to reduce the number of times people enter and exit and reduce the degree of pollution. The small window is 60cm wide, 40cm high and 30cm thick. The window sash is hung inside and outside.
(4) The volume of the sterile room is small and rigorous. After using for a period of time, the indoor temperature is very high, so a ventilation window should be provided. The ventilating window should be placed on the ceiling at the entrance of the inner chamber (that is, away from the worktable). It is a double-layer structure, the outer layer is a blind, and the inner layer can be a sash. The ventilating window can be opened after use in the inner chamber and before sterilization to circulate air, and the temperature can be installed under conditions.