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Stem cell laboratory construction

Aug 29, 2018

The layout of the laboratory is the basis of the laboratory design. Only the layout planning according to the functional partition and workflow requirements can ensure the subsequent professional design of water, electricity and wind. It can be said that the whole body is moving. Therefore, the layout design phase should consider the work and development needs as much as possible, rationally configure the space, and optimize the integration as much as possible. In addition to the optimization of the layout and the design of the position of the equipment, it should also fully consider whether the direction of the flow of people and the flow of goods meets the requirements of the work. For example, in order to avoid frequent running of the experimenter, the pre-processing room should be on the same floor as the instrument room; the gas cylinder room should be on the same floor as the gas chromatograph, GC/MS, etc.; try to maximize the single-layer area to avoid There are too many floors, and each floor should be equipped with a washing room and a sample room to make full use of the space; the biosafety room should reasonably arrange clean areas, semi-clean areas, and contaminated areas to avoid cross-contamination.


In the process of building a professional laboratory, the “construction” part is essential. It includes the positioning and planning of the laboratory, which is the foundation and soul of the entire laboratory construction. Laboratory positioning refers to: the positioning of the laboratory in the international and domestic (what kind of level should be achieved); the positioning of the laboratory in the industry (system); the positioning of the laboratory in the society (the third-party detection of the government function) Laboratory) The location of the laboratory in its geographic area. Laboratory planning includes two aspects: one is laboratory building planning, and the other is laboratory process planning. The laboratory building plan includes the appearance, style, height, and layout of the building. Before the construction of the laboratory, the process of investigating the needs of the laboratory is actually to determine the process of planning the entire laboratory for 3 to 5 years. Therefore, a large amount of preliminary research work is required. It is necessary to clarify its own needs and future development direction, and to extensively examine the established laboratories of relevant units and learn their experiences and lessons.


Stem Cell Laboratory - Culture Vessel


Commonly used cell culture vessels include culture flasks, culture plates, culture dishes, etc. The usual preparation amount is three times the amount used. The utensils should be made of materials with good transparency, non-toxicity, which are good for cell adhesion and growth, and commonly used disposable polystyrene materials. Products or neutral hardness glass products. Commonly used utensils are as follows:


  (1) Liquid storage bottle: used to store various prepared culture liquids, serum and other liquids. Commonly used specifications are 500ml, 250ml, 100ml and so on.


  (2) Culture flask: Different culture flasks have different shapes depending on the type of cultured cells. The cells used for cell subculture need uniform bottle wall thickness, which is convenient for cell adherence growth and observation. The size of the bottle mouth should be the same, and the caliber is generally not Less than 1cm, allow the straw to reach any part of the bottle, specifications 200ml, 100ml, 50ml, 25ml, 10ml and so on.


(3) Petri dish: used for open culture and other purposes. It is divided into several diameters of 30mm, 60mm, 120mm and so on.


(4) Straw: Commonly used are long straws and short straws. Long straws are also called scale straws. The modified tube has a spherical scale called improved straw, and a graduated straw is used to move liquid. It is commonly used in 1ml and 10ml. The short straw is also called the dropper, which is divided into two types: elbow and straight.


(5) Centrifugal tube: The centrifuge tube is the most widely used vessel in cell culture. It is used in various types according to the application. The centrifuge tubes commonly used for cell culture include large-abdominal sharp-bottomed centrifuge tubes and common sharp-bottomed centrifuge tubes. The former 50ml, 30ml, 15ml respectively; the latter is mostly 10ml and 5ml.


  (6) Others: such as flasks, beakers, measuring cylinders, funnels, syringes, etc.


Stem Cell Laboratory - Cell Culture Temperature


To maintain the vigorous growth of cultured cells, there must be a constant and appropriate temperature. Different types of cells have different culture temperature requirements. The standard temperature of human cell culture is 36.5 °C ± 0.5 °C. Deviating from this temperature range, the normal metabolism of cells will be affected. Influence, even death. The tolerance of cultured cells to low temperature is stronger than that of high temperature. When the temperature rise does not exceed 39 °C, cell metabolism is proportional to temperature; human cells can be damaged at 39-40 °C for 1 hour, but It is still possible to recover; at 40-41 ° C for 1 hour, the cells will be generally damaged, only a small half may recover; at 41-42 ° C for 1 hour, the cells are severely damaged, most of the cells die, and some cells still recover; When the temperature is above 43 °C for 1 hour, all the cells die. On the contrary, when the temperature is not lower than 0 °C, it has an effect on cell metabolism, but it has no harmful effect; when the cells are placed at 25-35 °C, the cells can still survive. And growth, but slow down; after a few hours at 4 ° C, and then returned to 37 ° C culture, the cells can continue to grow. Cell metabolism slows down with temperature. When the temperature drops below the freezing point, the cells can cause cells The icing is damaged and dies. But if Were added with an amount of cryoprotectant (glycerol or dimethylsulfoxide), or may be as -80 ℃ -196 deg.] C (liquid nitrogen) in a long-term cryogenic storage.