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What are the requirements for P4 laboratory construction?

Jan 23, 2019

The P4 laboratory is also a laboratory at the level of biosafety research. This level of experiment requires the handling of dangerous and unknown pathogens that may cause aerosol-borne pathogens or pose a high level of personal risk, and the pathogen still does not have any known vaccines or treatments, such as Argentine hemorrhagic fever and Congo Heat, Ebola, Marburg virus, Lhasa fever, Crimean-Congo haemorrhagic fever, smallpox, and various other bleeding disorders.

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Based on the degree of hazard in the P4 laboratory, the construction and management of the P4 laboratory must meet the following requirements:

1. Safety precautions must be strictly set in the P4 laboratory.

Positive pressure protective clothing with independent oxygen supply must be mandatory and must be used in the treatment of such biohazardous pathogens in the P4 laboratory. The four entrances and exits of the biological laboratory will be equipped with multiple showers, vacuum chambers and UV light chambers, as well as trace safety precautions that can destroy all biohazards.

Second, the P4 laboratory air and water supply should be strictly disinfected according to requirements.

Positive pressure protective clothing with independent oxygen supply must be mandatory and must be used in the treatment of such biohazardous pathogens in the P4 laboratory. The four entrances and exits of the biological laboratory will be equipped with multiple showers, vacuum chambers and UV light chambers, as well as trace safety precautions that can destroy all biohazards.

All air and water supplies in the P4 laboratory are subject to the disinfection procedures required by Biosafety Level 4 (or P4) laboratories and meet the same level of requirements to eliminate the possibility of accidental release. During the P4 experiment, it was found that when a pathogen is suspected or may have anti-protection laboratory medicinal properties, it must be processed in the BSL-4 laboratory until sufficient data is confirmed and must be continued in this specification laboratory or transferred to A lower level laboratory.

Third, the quality training of P4 staff must reach the level of professional experts and have the guidance of the centralized scientists.

P4 laboratory staff must have specific and in-depth training and understanding of standards and special measures, containment and design features for them to deal with extremely dangerous contagiousness. Staff are required to be professionally trained and qualified to handle these pathogens and to be supervised by the appropriate scientists. Access to the P4 laboratory is strictly controlled by the competent laboratory or supervisor.

Fourth, the location of the P4 laboratory must meet the requirements of hazard control.

The P4 laboratory must be in a separate building or building within the control area and completely isolated from other buildings in the area. And P4 laboratories must establish pollution prevention protocols often using negative pressure settings and preparing or using a specific equipment operation manual, so that even if the laboratory is damaged, it will seriously inhibit the outbreak of aerosol-borne pathogens. All activities in the field of work are limited to Class III biosafety cabinets or Class II biosafety cabinets and can only be used by persons with life support systems and positive pressure ventilation.

P4 laboratory is the most demanding experimental site in biosafety experiments, and it is also the laboratory with the highest level of protection in academic work such as biological subject research and experiment. The P4 laboratory is a laboratory at the level of biosafety research. Therefore, in the design, planning, construction, construction, we must strictly abide by the standards and the requirements of the use of management departments!