Cell culture laboratories are designed to prevent microbial contamination and harmful effects, requiring laboratory work environments to be clean, fresh air, dry and smokeless. Therefore, the cell culture laboratory should pay special attention to the planning and design process.
First, the laboratory design
1. Cell culture is an aseptic technique that requires the working environment and conditions to be free from microbial contamination and other harmful factors.
2, cell culture room and design principles are to prevent microbial contamination and harmful factors, requiring a clean working environment, fresh air, dry and smokeless dust.
3. The design principle of the cell culture room is generally that the aseptic operation area is located inside the room where the room is less moving, and the conventional operation and the closed culture are in one room, which is a clean room of a 100,000-level clean environment. The scrubbing and disinfecting work is located outside the clean room to avoid contamination of the indoor environment. The cell clean room requires an air conditioning system and maintains a certain positive pressure on the outside.
4, the anatomy laboratory is mainly used for the separation of organ tissues, need to have aseptic table, dissection microscope, various surgical equipment.
Second, laboratory equipment
1, ultra-clean workbench / biological safety cabinet
Cell culture needs to be in a sterile environment. When performing cell experiments, it needs to be carried out in air at level 100. Then we need to purchase a clean bench or biosafety cabinet that can provide 100 levels of cleanliness.
For most laboratories, the ultra-clean workbench is fully capable of meeting the laboratory's operational needs. As for the size of the ultra-clean platform, it depends on the size of the laboratory and the number of people to determine whether it is a single or a double, whether to buy one-sided or two-sided, and buy a few. If the experimental operation is biohazardous, you should choose a more advanced biosafety cabinet than the ultra clean bench.
2, carbon dioxide incubator
Carbon dioxide incubators have been widely used in most cell culture chambers. The advantage of the carbon dioxide incubator is that it can provide a certain amount of CO2 (usually 5%) required for cell culture, and it is easy to stabilize the pH of the culture solution, and is suitable for open or semi-open culture.
Inverted microscopes are essential equipment in the daily work of cell culture laboratories. They are mainly used to understand the growth of cells and observe the presence or absence of pollution. If funds permit, it is recommended to use a high-quality phase contrast microscope, a dissecting microscope, a fluorescence microscope, a video system or a time-lapse filming device equipped with a camera system to record and record cell growth at any time.
4, pure water machine / ultra pure water machine
Cell culture also requires ultrapure water system. Ordinary pure water and distilled water can not be used for cell culture, while ultrapure water is mainly used to configure some medium and reagent. The water of ultrapure water can be tap water or secondary pure. Water (good quality deionized water, resistance of about 10 megohms), in addition to ultra-pure cell culture water, the most important endotoxin has strict requirements, so when selecting ultra-pure water machine, it is necessary to select ultra-filter components. Model.
Cell culture requires centrifugation to collect passage cells. The general rotation speed is between 1000 and 1500 rpm, so it is necessary to purchase a low-speed normal temperature centrifuge, and also bring 10 ml, 50 ml and the rotor of the culture plate. A high-speed refrigerated centrifuge is also required to collect cell pellets or cell supernatants.
6, liquid nitrogen tank
To establish a cell bank, a liquid nitrogen tank is necessary. The frozen cells in a liquid nitrogen tank can generally ensure biological activity in the past ten years. As for the size of the liquid nitrogen tank, according to the size of the cell bank. And set.
7, low temperature refrigerator
The cells need to be stored at -70 to -80 degrees overnight for cryopreservation and then placed in a liquid nitrogen tank, which requires a low temperature freezer.
8, the transfer window
The cell house should be isolated from the outside world when it is constructed. This requires purchasing a transfer window to deliver the item, reducing the chance of contamination, and all the items entering the cell room are UV-sterilized in the transfer window to ensure the safety of the clean room.
All kinds of cell laboratory utensils need to be sterilized before and after use. The sterilization conditions are generally 121 ° C for 20 to 30 minutes. This requires an autoclave placed in a clean room to ensure biosafety.
10, pipetting gun, electric pipetting gun
In the cell experiment operation, various tips and pipettes are required, so it is necessary to configure the pipette.
11, all kinds of consumables needed in cell culture
Various cell culture plates, cell culture flasks, plate pipettes, etc.
12, the rest of the instrument
For cell experiments, oscillators and magnetic stirrers may also be required for mixing liquids; some non-cleanable stainless steel or glassware containers filled with cells require the help of an ultrasonic cleaner; if immunological experiments are involved, ice is definitely required. If the demand for bath and crushed ice is large, you need to buy an ice machine. In addition, the broken cells may require an ultrasonic cell disrupter. Finally, if the cell house is large, a trolley must be provided. Liquid media is always a tormenting thing.