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How Should A PCR Laboratory Be Designed?

Jul 17, 2018

The main reason for PCR laboratories is to avoid cross-infection between laboratories. Therefore, the requirements for sending and exhausting air are particularly important. So, how should PCR labs be designed? Next, we will introduce them to VOLAB. The design of the PCR laboratory:

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The PCR laboratory can be divided into two large functional work areas: the pre-nuclear amplification region and the nucleic acid amplification region.

The pre-amplification area includes the reagent preparation room and the sample preparation room. The two rooms are independent of each other and air intercommunication cannot occur. The two rooms are in a positive pressure state relative to the outside air pressure. The post-nuclear acid amplification region includes an amplification region and a product analysis region, which can be combined into one room using a real-time fluorescence PCR instrument, a PCR laboratory of an HIV viral load meter. If this area is divided into two rooms, the two rooms must also be independent of each other, and air intercommunication should not occur. The post-amplification zone is under negative pressure to the outside air pressure.

The PCR laboratory sets the working areas according to the function of the instrument. For example, the polymerase reaction is used: four separate working areas of the reagent storage and preparation area, the sample preparation area, the amplification area, and the amplification product analysis area are set. Is the most commonly used partitioning method. If the sample needs to be pulverized, it is necessary to add a sample crushing zone; if real-time fluorescent PCR is used: the gene amplification zone and the gene product analysis zone can be combined in one room; using sample processing, nucleic acid extraction and amplification detection as one. In an automated PCR analyzer, the sample preparation area, the amplification area, and the amplification product analysis area can be combined into one area, so the PCR laboratory is in principle set up with five or four zones or three zones or two separate workspaces. .

Laboratory equipment is also an indispensable accessory for the work. In the design of the PCR laboratory, different instruments and equipment should be provided according to the requirements of each laboratory, such as: centrifuge, biological safety cabinet, ultra-clean workbench, and sample loading. , electrophoresis (slot), electrotransfer, hybrid or hybridization box, water bath, DNA sequencer, microplate reader and plate washer.

As a molecular biology technology, PCR laboratory can amplify DNA fragments and use the DNA gene tracking system to detect the virus content of the human body more quickly and accurately. It is a reliable basis for medical treatment and brings advanced, A quick and efficient medical basis.

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