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Microbial Laboratory Layout Design

May 21, 2018

The purpose of the microbiology laboratory layout design is "people flow, logistics, sewage flow through different channels, unidirectional circulation, sterilization of the culture medium and waste sterilization space, to minimize cross-contamination, set up the sample temporary storage, to ensure the continuation of the sample state Sex and stability are conducive to the accuracy of the test results.Microbial laboratory layout design shown in Figure 1.

1 Microbiology laboratory layout design sketch

The flow of people enters and exits the sterile room through the first-level buffer room and the earphone buffer room. The door locks of the clean rooms are all interlocked structures, which ensures the isolation of the environment of the sterile room and the external environment. The culture medium, operating tools and samples enter the sterile room through the buffer window. After the operation is completed, the culture window is accessed through the buffer window on the other side for culture and identification. The buffer window is an interlocking structure.

After the identification and identification of the waste, the contaminated stream is directly placed in the hazardous waste sterilization room and sterilized, and the waste is treated as harmless according to medical waste standards.

2, equipment, reagent supplies procurement

The procurement of microbiological laboratory equipment and reagent consumables is determined according to the project to be carried out by the laboratory, and the main equipment for the routine is shown in the table.

It should be noted that the volume of the sterilizer is not more than 100L. It is a simple pressure vessel and does not require a professional pressure vessel operation certificate. Attention to this point when purchasing equipment can reduce many issues for the laboratory. Precise constant-temperature water bath is an essential equipment. When testing E. coli and fecal coliforms, it must be incubated in a water bath. The temperature control accuracy of the incubator cannot meet the experimental requirements.

The microbiology laboratory is designed as a fully enclosed space. The surrounding spaces and the upper floors are made of colored steel plate structure. The filling material is fire-retardant material. The main body color is dull white, and the door and window glass is common glass. To prevent deposition dust, the window material uses R25MM aluminum alloy round Arc pressure line

All the two-dimensional joints use the R50MM aluminum alloy fillet on the inner side, and the outer corners of the two-dimensional connecting line are connected with the outer corner of the R100MM aluminum alloy. The three-dimensional joints of the color steel plate use excessive three-dimensional joints, while the color steel plate and the corner floor are connected by aluminum alloy tanks. The ceiling material is also a color steel plate, and the floor is made of epoxy resin material, which is characterized by no gap, corrosion resistance, smoothness, and easy cleaning. The ground skirting is decorated with shaded aluminum, which is beautiful and rigorous. The entire laboratory should form a sterilized, seamless, smooth, beautiful, non-reflective, dust-free, rust-free, moisture-proof, antibacterial, and high-performance sterile surface and inner shell.

3, Biosafety Laboratory Building Specifications

It will be implemented from day one. The implementation of this standard has changed the long-term lack of a unified standard for biosafety laboratory construction and construction technology in China. The content of the specification includes the technical requirements for the construction plane, decoration and structure of biosafety laboratories, the basic technical specifications of the laboratory, air conditioning and air purification, water supply and drainage, gas supply, distribution, automatic control and firefighting facility configuration and construction, Acceptance and testing principles, methods and other aspects. It is suitable for the construction, reconstruction, and expansion of microbiology, biomedicine, animal experiments, genetic recombination, and biological products.

The design, construction and acceptance of physical safety laboratories, and the establishment of biosafety laboratories shall be based on biosafety to ensure the safety of laboratory personnel and the safety of the surrounding environment of the laboratory, and at the same time protect the subjects from being contaminated according to experimental needs. In architecture should be based on practical and economic principles.

In order to strengthen biosafety work in veterinary laboratories, prevent the spread of animal pathogenic microorganisms, ensure the control and extermination of animal epidemics, and ensure the safety of animal husbandry, the Ministry of Agriculture has issued the “Management of Animal Epidemic Prevention Law of the People's Republic of China” and the “Administrative Measures for Review of Animal Epidemic Prevention Conditions”. The relevant regulations refer to the interim administrative measures of the International Laboratory for Infectious Atypical Pneumonia Viruses related to laboratory biosafety.

SARS is a serious infectious disease. In order to ensure bio-safety, prevent experimental personnel, infect and pollute the environment, the Ministry of Science and Technology has organized the provisional management of the SARS Virus Research Laboratory.

This method implements a hierarchical management of laboratories engaged in research on SARS virus. The laboratories are divided into: atypical pneumonia virus laboratory, infectious atypical pneumonia virus infection animal laboratory, and infectious atypical pneumonia virus infection animal laboratory.

This method clearly stipulates the management requirements for the organization, management, rules and regulations, and health care supervision of the laboratory.