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PCR Laboratory Construction Planning Considerations

Sep 14, 2018

Laboratory construction is not a small matter, sloppy, laboratory construction planning is relatively cumbersome, at the same time, it should pay attention to a lot of things, if it is a little careless, there will be leakage, resulting in experimental accidents, then PCR experiment What should I pay attention to in the construction of the room?

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1. Main structure:

The main body is color steel plate and aluminum alloy profile. All the inner and inner corners of the room are made of aluminum alloy 50 rounded aluminum, which solves the problems of easy pollution, dust accumulation and difficult cleaning. Firm structure, simple lines, beautiful

Generous, good sealing.

2. Standard three-zone separation and air pressure adjustment:

The PCR process was divided into three separate experimental zones: reagent preparation, specimen preparation, and PCR amplification. There is an overall buffer corridor throughout the area. Each independent experimental area is provided with a buffer zone, and each zone passes

Air pressure regulation protects reagents and specimens from aerosol contamination throughout the PCR experiment and reduces contamination of personnel and the environment by amplification products.

The buffer can be opened, the exhaust fan of the buffer and the PCR amplification area is exhausted outside, and the air outlet with adjustable air volume is installed on the outer wall of the experimental area and each door, and the air is ventilated indoors through the return air outlet.

3. Disinfection:

UV lamps are installed in the three experimental areas and on the top of the three buffers and inside the transfer window for disinfection.

A moving ultraviolet lamp is also provided in the reagent preparation area and the sample preparation area to partially disinfect the experimental table.

4. Mechanical chain stainless steel transfer window:

Reagents and specimens are delivered through mechanical interlocking stainless steel (not recommended for electronic interlocking) to ensure that reagents and specimens are not contaminated during transfer (personal shunt)

5, the ground

It is recommended to use PVC coiled floor or self-flowing floor for good ground. Easy to clean and corrosion resistant. If there are no conditions, you can also use terrazzo floor, or large tiles (at least 800mm × 800mm)

The seam needs to be less than 2mm.

6, lighting

The lamps should be cleaned and sleek, which can be easily cleaned and dust-free.