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What Are The Key Points Of PCR Laboratory Construction?

Sep 15, 2020
  1. Main structure


The main body is clean color steel plate and aluminum alloy profile. The inner and outer corners of the room are made of aluminum alloy 50 inner rounded aluminum, which solves the problems of easy pollution, dust accumulation and difficult cleaning. The structure is firm, the lines are simple and beautiful, and the sealing performance is good.


2. Standard three-zone separation and pressure difference control

The PCR process is divided into three independent experimental areas for reagent preparation, sample preparation and PCR amplification and detection. There is an overall buffer corridor throughout the area. Each independent experimental area has a buffer zone.

PCR laboratory air-conditioning ventilation system design and pressure control PCR laboratory does not have strict purification requirements, but in order to avoid the possibility of cross-contamination between various experimental areas, it is advisable to adopt the air flow organization form of full delivery and full exhaust. At the same time, the ratio of air supply and exhaust must be strictly controlled to ensure the pressure requirements of each experimental area.


3. Disinfection

Ultraviolet lamps are installed in the three experimental areas, the top of the three buffer zones and the transfer window for disinfection. In the reagent preparation area and the specimen preparation area, mobile ultraviolet lamps are also installed to locally disinfect the laboratory bench.

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4. Mechanical interlocking stainless steel transfer window

Reagents and specimens are transmitted through a mechanically interlocked stainless steel (electronic interlocking is not recommended) transmission window to ensure that the reagents and specimens are not contaminated during transmission (personal shunt).


5. Ground

The floor generally adopts PVC coiled material floor or artesian floor, which has good integrity. Easy to clean and corrosion resistant. You can also use terrazzo ground, or large tiles (at least 800mm×800mm) and the joints need to be less than 2mm. Try to avoid dust generation and dust accumulation.


6. Lighting

The lamps should be purified lamps, which are easy to clean and dust-free.