Microbiology laboratories are mainly used for microbial purification, separation, proliferation and identification in aseptic environments, and have high requirements for aseptic environment. So what equipment is needed in the microbiology laboratory?
First, the sterile room
The sterile room is the core part of the laboratory and provides protection for the sample to ensure accurate results and safety.
Second, the ultra-clean workbench
Microbial cultures are all cultured in a specific medium, so sterile cultures necessarily require a clean bench to provide a sterile working environment. The main use of the clean bench is the inoculation of microorganisms and the aseptic processing during handling.
What are the instruments and equipment used in the microbiology laboratory?
Third, high pressure steam sterilization pot
The autoclave is a closed double metal pan that can withstand a certain pressure. Generally, it is necessary to extrude the operating vessel, the culture medium, and the like in an autoclave before performing the aseptic operation.
Fourth, the incubator
The incubator is mainly used for the cultivation of laboratory microorganisms, providing a suitable environment for the growth of microorganisms. The incubator has the following types:
1, ordinary incubator
2, biochemical incubator
3, constant temperature and humidity box
4, anaerobic incubator
Five, the balance
The balance is a device for accurately weighing various reagents and drugs. Commonly used in laboratories are electronic balances.
First, meaning and purpose
Microbiology is the science of studying microbes and their life activities. Microorganisms have many kinds of characteristics such as various types, small volume, many types of metabolism, high metabolic intensity, fast growth and reproduction, and easy variation. It is widely distributed in air, water, soil, human body, animal and plant, and it is independent or parasitic. Most microorganisms are beneficial to humans, animals and plants, especially because they have a close relationship with human life and have a great impact on industrial and agricultural production, human living environment and health. With the new curriculum reform, the number of middle school microbes in secondary school teaching has also increased, especially the "microbial laboratory training" experiment is a subject experiment of the human science edition of the standard high school curriculum standard experimental textbook biological selection I. Microbiology experiments are not only comprehensive and operative, but also require students to have strong aseptic skills and biosafety awareness. It is of great significance to train students' theory, practical ability, ability to analyze and solve problems, and scientific thinking.
Second, function and requirements
The main equipment of the microbiology laboratory are: fermenter, autoclave, ultra-clean workbench, electrothermal constant temperature incubator, constant temperature and humidity incubator, electric blast drying oven, pipette, Olympus microscope, refrigerator, Modern high-tech experimental equipment such as full-temperature oscillator and far-infrared quick drying oven. The laboratory can carry out microbial culture, production and separation of microbial metabolites, detection of microbial indicators for food and drinking water, and screening of microorganisms. It can better complete the teaching experiment of teaching materials, and the modern instrument configuration can satisfy the students' interest in inquiry activities.
Design The microbiology laboratory consists of a preparation room, a washing room, a sterilization room, a sterile room, a constant temperature culture room, and a general laboratory. The common feature of these rooms is that the texture of the floor and walls is smooth and hard, and the instruments and equipment are simply furnished for easy cleaning.
Second, the basic requirements of the microbiology laboratory A preparation room preparation room for the preparation of culture media and sample processing. Indoors are equipped with reagent cabinets, storage cabinets or materials for counters, test benches, electric stoves, refrigerators, water and sewage, power supplies, etc.
The washing chamber washing chamber is used for washing dishes and the like. Pathogenic microorganisms are sometimes present because used utensils have been contaminated with microorganisms. Therefore, the washing chamber is set up as conditions permit. Indoors should be equipped with heaters, steamers, basins and buckets for washing utensils, as well as various bottle brushes, decontamination powder, soap, washing powder, etc.
Third, the sterilization room sterilization room is mainly used for sterilization of the medium and sterilization of various instruments. The room should be equipped with high-pressure steam sterilizer, oven and other sterilization equipment and facilities.
4. Sterile room The sterile room is also called the inoculation room. It is a special laboratory for aseptic operation such as system inoculation and purification of bacteria. In the microbe, the inoculation transplantation of the strain is a major operation. The characteristic of this operation is to ensure the pure species of the strain and prevent the contamination of the bacteria. In the air of the general environment, due to the presence of many dust and bacteria, it is easy to cause pollution and interferes greatly with the inoculation work.
1. Setting up the sterile room The sterile room should be set according to the principles of economy and science.
The basic requirements are as follows:
(1) The sterile room should have two inner and outer rooms, the inner room is a sterile room, and the outer part is a buffer room. The volume of the room should not be too large to facilitate air sterilization. The small inner area is 2×2.5=5m2, the outer area is 1×2=2m2, and the height is 2.5m or less. All should have ceiling.
(2) Sliding doors shall be provided in the interior to reduce the fluctuation of the air. The door shall be located at a position far from the workbench; the outer door shall also be used for sliding doors, and shall be located at a distance from the inside.
(3) A small window shall be opened on the wall or "sparing fan" between the inner and outer compartments to make the necessary passages for the inside and outside of the inoculation process to reduce the number of times people enter and exit and reduce the degree of pollution. The small window is 60cm wide, 40cm high and 30cm thick. The window sash is hung inside and outside.
(4) The volume of the sterile room is small and rigorous. After using for a period of time, the indoor temperature is very high, so a ventilation window should be provided. The ventilating window should be placed on the ceiling at the entrance of the inner chamber (that is, away from the worktable). It is a double-layer structure, the outer layer is a blind, and the inner layer can be a sash. The ventilating window can be opened after use in the inner chamber and before sterilization to circulate air, and the temperature can be installed under conditions.